Plant Tissue Culture Mastery

Explant: Any part of the plant (leaf segment, stem tip, root section, anther, single cell, protoplast) isolated from its original location and used to initiate a culture in vitro .
Callus: An undifferentiated, disorganized mass of proliferating cells, usually formed when an explant responds to high levels of specific plant growth regulators. Callus is the starting material for several regeneration pathways.
Dedifferentiation: The process whereby mature, specialized cells (e.g., phloem cells) revert to a meristematic state and regain the capacity for cell division.

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